[Histonet] PAN CKMNF116, picking up dendritic cells?

[Richard Cartun]

It should label dendritic cells since it reacts with cytokeratin 8.  These cells have been shown to contain cytokeratin protein by immunoblotting.  I use this reactivity as an internal positive control.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596
(860) 545-2204 Fax

>>> jAswigueAlatan <msjaswigue.alatan <@t> gmail.com> 9/24/2012 9:14 PM >>>
Dear Histonetters,
Has anyone out there experienced problem with optimizing PanCK MNF116
antibody? Our CKMNF116 stains dendritic cells on lymph nodes using Ventana
protocol:
CC1 - 8 mins,
Ab incubation time 16 mins,
Post primary peroxidase inhib selected.
Basically, it should stain cytokeratins, but it stains dendritic cells as
well. What would be the possible causes and solutions. Any suggestions
would be greatly appreciated. Many thanks.

Joy
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