[Histonet] PAN CKMNF116, picking up dendritic cells?

[jAswigueAlatan]

Dear Histonetters,
Has anyone out there experienced problem with optimizing PanCK MNF116
antibody? Our CKMNF116 stains dendritic cells on lymph nodes using Ventana
protocol:
CC1 - 8 mins,
Ab incubation time 16 mins,
Post primary peroxidase inhib selected.
Basically, it should stain cytokeratins, but it stains dendritic cells as
well. What would be the possible causes and solutions. Any suggestions
would be greatly appreciated. Many thanks.

Joy