[Histonet] methanol vs ethanol in processing

Rene J Buesa rjbuesa <@t> yahoo.com
Thu Sep 20 09:08:40 CDT 2012

Probably not but processing with methanol is a very bad idea. It is highly toxic and tends to make tissues somewhat brittle.
René J.

From: Michelle Perrins <Michelle.Perrins <@t> uct.ac.za>
To: histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, September 20, 2012 8:46 AM
Subject: [Histonet] methanol vs ethanol in processing

Hello all

If tissue (from autopsy cases) has been fixed in 10% formal saline,
will there be a difference in the  stained section if:
processed in methanol
processed in ethanol


Michelle Perrins
Chief Medical Technologist
Forensic Pathology Services
Division Forensic Medicine 
Faculty Health Sciences
University of Cape Town

tel: +27 21 406 6001
fax: +27 21 448 1249
Email: Michelle.Perrins <@t> uct.ac.za



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