[Histonet] RE: m endothelial cell in FFPE

Reynolds,Donna M dreynold <@t> mdanderson.org
Fri Sep 14 12:26:03 CDT 2012

I recently tested a mCD34 from GeneTex cat GTX28158 that gave very nice labeling of mouse endothelial cells on FFPE. Used EDTA pH 8 antigen retrieval and no amplification.
For those of you using the Dianovo mCD31 antibody what are you using for antigen retrieval? And are you using amplification? I tried this antibody a year or two ago and was not very happy with the results.
I have also used the VonWillebrand. It give very nice label of large established vessels but not the new or smaller vessels. 
Donna Reynolds HT(ASCP
Chief Histology Tech, Cancer Biology IHC Research Lab
M.D. Anderson Cancer Center Houston, TX

Message: 1
Date: Thu, 13 Sep 2012 13:05:37 -0600
From: "Patsy Ruegg" <pruegg <@t> ihctech.net>
Subject: RE: [Histonet] endothelial cell marker
To: "'Amos Brooks'" <amosbrooks <@t> gmail.com>,
	<histonet <@t> lists.utsouthwestern.edu>,
	<joost.bruijntjes <@t> tno.triskelion.nl>
Message-ID: <D9F8AB2BD9D14EF783339ABC4BD9A0CF <@t> DESKTOP3>
Content-Type: text/plain;	charset="us-ascii"

By far the best ab for endothelial cells in mouse tissue is rat anti CD31 from Dianovo, it picks up the really early forming vessels better than any other cd31 or F8 or SMA I have ever used.  We like it in AP/red.


     My favorite by far is VonWillebrandt (spelling?) factor VIII. Dako has a really good one (Cat# A0082) that works great in mouse tissue. CD31 is OK but it is really finicky. CD34 works but F8 is easier and more reliable.


On Wed, Sep 5, 2012 at 1:01 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 5
> Date: Wed, 5 Sep 2012 10:04:58 +0000
> From: "Bruijntjes, J.P. (Joost)" <joost.bruijntjes <@t> tno.triskelion.nl>
> Subject: [Histonet] endothelial cell marker
> To: "Histonet <@t> lists.utsouthwestern.edu"
>         <Histonet <@t> lists.utsouthwestern.edu>
> Message-ID:
> <A3A9D8B3F110D44FA1022B49913668668835BE43 <@t> EXC-MBX03.tsn.tno.nl>
> Content-Type: text/plain; charset="us-ascii"
> Hi all
> Is anyone of you familiar with an antibody directed against 
> endothelial cells which can be applied on FFPE mouse tissues?
> Best regards
> Joost Bruijntjes
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