[Histonet] mouse testis in Bouins

[Geoff]

Fix for a week or two, then rinse out most of the picrates with 50-70% 
ethanol. Fixation preserves cell morphology, it does not distort it.

Geoff

On 9/14/2012 9:06 AM, Margaret Horne wrote:
>   Hello Everyone, I am asking this for a friend.
>   
> How long can mouse testis be kept in Bouins without distortion of cell
> morphology? Days? weeks? months? years?
>   
> I noticed in the Archives that many people fix in Bouins , rinse, then
> store in 70% EtOH. This is preferable I assume. Again, how long is ok?
>   
>                                                          
>     Thanks in advance for the sharing of your accumulated wisdom,
>                            Margaret
>   
>
>
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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voice: (732)-235-4583; fax: -4029
mcauliff <@t> umdnj.edu
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