[Histonet] air drying special stain slides rather than dehydrate and clear

[Amos Brooks]

Hazel,
The only time I have seen any problem was when a stain was not fully washed
off the slide. Once you put the slide in xylene and coverslip it the slide
is just like any other.

Amos
On Sep 12, 2012 8:57 AM, "Horn, Hazel V" <HornHV <@t> archildrens.org> wrote:

> I suppose my age will show here but I was always taught to NEVER let
> slides dry out unless the procedure indicated such.   Is there no drying
> artifact when you let these slide dry before coverslipping?
>
> Hazel Horn
> Supervisor of Histology/Autopsy/Transcription
> Anatomic Pathology
> Arkansas Children's Hospital
> 1 Children's Way | Slot 820| Little Rock, AR 72202
> 501.364.4240 direct | 501.364.1302 office | 501.364.1241 fax
> hornhv <@t> archildrens.org
> archildrens.org
>
>
>
>
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> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:
> histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amos Brooks
> Sent: Tuesday, September 11, 2012 7:31 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] air drying special stain slides rather than dehydrate
> and clear
>
> Hi,
>      My choice to air dry rather than dehydrate in ETOH & xylene is based
> on the stain rather than the spooky xylene hazard boogyman. Yes, not using
> xylene if it is not really needed is not a bad idea, but the main reason I
> air dry some stains is the alcohols remove some of the stains. Ever have a
> beautiful Luxol Fast Blue bleach out on you? The most exasperating thing in
> the world!
>     Generally stains that end in water can easily be air dried. Something
> alcoholic like eosin or Movat's Pentachrome ending in alcoholic saffron
> might as well be finished traditionally. I air dry any stain that is
> counterstained in Nuclear Fast Red, Light Green, Methyl Green. I have air
> dried IHCs with no ill effects too. Don't try it with fluorescents though,
> that would be bad ... and pointless.
>      I don't put them in an oven. I set them at the front of the fume hood
> and go do something else for a few minutes. If I want to rush it I close
> the sash to increase the flow rate for a bit. (Of course it is opened back
> up right after so the draft works properly.) Amos
>
>
> On Tue, Sep 11, 2012 at 11:09 AM, <
> histonet-request <@t> lists.utsouthwestern.edu
> > wrote:
>
> > Message: 16
> > Date: Tue, 11 Sep 2012 10:32:08 -0400
> > From: "Diana McCaig" <dmccaig <@t> ckha.on.ca>
> > Subject: [Histonet] air drying special stain slides rather than
> >         dehydrate       and clear
> > To: <histonet <@t> lists.utsouthwestern.edu>
> > Message-ID:
> >         <DCFD9E6A390E294AAF3A2561CD32E5C417A90529 <@t> ckhamail1.ckha.on.ca>
> > Content-Type: text/plain;       charset="us-ascii"
> >
> > I was hoping to get information on why special stains are dehydrated,
> > cleared and mounted vs allowing them to be blotted dry, air dried then
> > coverslip.
> >
> >
> >
> > Every procedure I have ever encountered always indicates to dehydrate
> > and clear but I have heard where some labs are blotting the slides ,
> > allowing to air dry (probably not set standard time) and dipped in
> > xylene prior to cover slipping.  Reason given is that the counterstain
> > gets washed out.  Wouldn't adjusting the times be a better resolution.
> >
> >
> >
> > I understand residual water could be present and cause long term
> > issues on storage but wanted some other opinions on this process.
> >
> >
> >
> > Diana
> >
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