[Histonet] RE: CD11c

[McMahon, Loralee A]

 Cell Marque has one that I have used in formalin fixed paraffin embedded tissues.  We use the Dako Flex detection system.  Antibody diluted to 1/50.  

Loralee McMahon, HTL (ASCP)
Immunohistochemistry Supervisor
Strong Memorial Hospital
Department of Surgical Pathology
(585) 275-7210
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of John Shelley [jshelley <@t> sanfordburnham.org]
Sent: Friday, October 26, 2012 1:35 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] CD11c

Happy Friday,

I have a request for anyone who has worked with CD11c from any vendor. I have tried at least two antibodies on mouse tissue both frozen and FFPE and have not been able to get results at all from FFPE and the frozen sample is hit or miss. My hope is that someone has a working protocol and is willing to share with me the details of how they were able to get it to work. I would prefer FFPE but frozen is an acceptable option. I appreciate in advance your consideration to my request. Thanks!!!!

Kind Regards!

John J Shelley

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