[Histonet] Fwd: MIXED fiber type identification by
liz <@t> premierlab.com
Mon Oct 22 13:20:09 CDT 2012
I think you normally use ATPase at different pH's, which is an enzyme stain, to differentiate muscle fibers.
At pH 10.2 it would stain Type I fibers light and then Type II Fibers dark (all types IIa, IIb and IIc) the other pH's stratify out the type II fibers.
Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
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From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of arjun ruhella
Sent: Monday, October 22, 2012 11:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Fwd: MIXED fiber type identification by Immunohistochemistry
Its been a while i asked for a doubt to all histology experts around US, but i have not been able to get much reply (only 1 :(). I thought my doubt/e mail might have been missed by some, so sending it again.
I would really appreciate if anybody could guide me in my problem.
Hope to hear people's advices this time.
Begin forwarded message:
> From: arjun ruhella <ruhella.arjun <@t> gmail.com>
> Date: October 2, 2012 8:34:59 AM EDT
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: MIXED fiber type identification by Immunohistochemistry
> Greetings to all,
> In immunohistochemistry, I am encountering confusion in designating muscle fibers (based on staining color intensity) to a particular fiber type.
> This problem is particularly bothersome when i encounter the fibers stained with 2 different colors, one color has good intensity while other seems to be of light intensity. I don't have any criterion whether to consider this light staining as actual staining, which would classify the fibers as MIXED fibers or should i consider this light staining as background/non specific staining in which case the fibers will be classified as PURE fibers.
> Does anybody can suggest any criterion/tips they have been using to identify muscle fiber type as correctly as possible? I can understand that identifying MIXED fibers is subjective in immunohistochemistry, but it would be of immense help if i could get to know of any objective suggestions.
> Animal= spinal cord injured Rat
> Muscle = Soleus
> Section Thickness = 10µm
> I will really appreciate the help!
> Thanks, hope to hear from ppl soon!
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