[Histonet] Fwd: MIXED fiber type identification by Immunohistochemistry

[arjun ruhella]

Dear all,

Its been a while i asked for a doubt to all histology experts around US, but i have not been able to get much reply (only 1 :(). I thought my doubt/e mail might have been missed by some, so sending it again.

I would really appreciate if anybody could guide me in my problem.

Hope to hear people's advices this time.
Thanks

Best,
Arjun
 

Begin forwarded message:

> From: arjun ruhella <ruhella.arjun <@t> gmail.com>
> Date: October 2, 2012 8:34:59 AM EDT
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: MIXED fiber type identification by Immunohistochemistry 
> 
> Greetings to all, 
> 
> In immunohistochemistry, I am encountering confusion in designating muscle fibers (based on staining color intensity) to a particular fiber type. 
> 
> This problem is particularly bothersome when i encounter the fibers stained with 2 different colors, one color has good intensity while other seems to be of light intensity. I don't have any criterion whether to consider this light staining as actual staining, which would classify the fibers as MIXED fibers or should i consider this light staining as background/non specific staining in which case the fibers will be classified as PURE fibers.   
> 
> Does anybody can suggest any criterion/tips they have been using to identify muscle fiber type as correctly as possible? I can understand that identifying MIXED fibers is subjective in immunohistochemistry, but it would be of immense help if i could get to know of any objective suggestions.
> 
> 
> Animal= spinal cord injured Rat
> Muscle = Soleus
> Section Thickness = 10µm 
> 
> I will really appreciate the help! 
> 
> Thanks, hope to hear from ppl soon! 
> 
> BEST, 
> 
> AR 

Best,
Arjun