[Histonet] Need help with spirochete control

[Sheila Fonner]

Hello all,
I am in need of some help with a spirochete protocol for the Leica Bond.  I am using a Biocare primary antibody.  I've tried ER1 for 20 min. with 15 min. Ab with no luck.  Does anyone do this, and do you think increasing the primary Ab time would help?  I've used this same Ab before on a different platform and had no problem.  Any help or advice would be greatly appreciated.  Thanks.
Sheila, HT (ASCP)