[Histonet] CAP GEN. 41770 Residual Detergent on Glassware

[Geoff]

Over the years I have heard about some folks obsessing (my opinion) 
about this.
I have never had a problem and I  don't think it is an issue, assuming 
one rinses the glassware thoroughly.
If the glass is clean water does not bead but sheets off. I only used 
distilled water for silver stains, making osmium for EM, etc.
Look up 'water break test' and see if you can find anything helpful.

Geoff

On 10/12/2012 11:40 AM, Valerie O'Connor wrote:
> Hi All,
> I process and stain GI biopsies only. I use do a basic H&E, Diff Quick for H.pylori, PAS for candida , Trichrome, and Alcian Blue/PAS.
> Is anyone aware of documentation stating that residual detergent on glassware would have a deleterious affect on the quality of the
> stains mentioned above. I cannot find anything.
> Thanks in advance,
>
> Valerie O'Connor B.S. HT (ASCP)
> Gastroenterology Center of CT.
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Geoff McAuliffe, Ph.D.
Neuroscience and Cell Biology
Robert Wood Johnson Medical School
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mcauliff <@t> umdnj.edu
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