[Histonet] Mixed fiber type identification
ruhella.arjun <@t> gmail.com
Thu Oct 4 13:17:28 CDT 2012
I really appreciate your suggestions. That being said, i really cannot go for mATPase or PAS stain, I need to do IHC.
I hope to hear from other too on this issue.
On Oct 4, 2012, at 1:20 PM, mtitford <@t> aol.com wrote:
> Arjun Ruhella asked about fiber typing of animal soleus muscle:
> No one answered him, at least in public. I guess all the hotshots are in Vancouver!
> Years ago I was involved in fiber typing and measuring of human muscle biopsy fibers. In those human muscle biopsies, all the bundles were mixed, about 60:40 if I remember correctly. The proportion and size of the fibers changed depending on the disease process.
> We used the ATP reaction on frozen sections to visualise the type 1 and type II, (and IIA, IIB, etc) fibers. Then, in those pre-computer days, we selected a typical muscle bundle and started counting the cross sectional diameter of those type I and type II fibers, using eypepiece graticule in the microscope. A histogram was prepared.
> I cannot imagine using IHC to demonstrate the type I and Type II fibers is a whole lot different, or that animals have muscle fibers that are not mixed.
> You can see some differentiation on a good PAS stain, but I do not know how well it compares.
> The procedure we used was in "Muscle Biopsy: A modern approach" by Dubrowitz and Brooke, Saunders. 1973 ( I told you it was years ago!)
> Hope this helps
> Michael Titford
> Pathology - USA
> Mobile AL
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