[Histonet] Staining for Osteoid in FFPE tissues

[Erickson, Jamie E]

Hello All,
Have anyone tried this for osteoid in paraffin. I understand plastic are the best but I can do plastics. Will this work or are there any other alternatives to getting osteoid in FFPE.  We use formic acid (cal-rite)  for decaling our process is; fix 24 Hrs then cal-rite 2 days then new cal-rite 2 days then wash thoroughly and process mouse Paws/tibia/vertebra.
This paper says osteoid is well differentiated by cyanuric chloride solution prior to decalcification (EDTA is better).
Has anyone tried this?
Jamie

IMPROVED PROCEDURE FOR HISTOLOGICAL IDENTIFICATION
OF OSTEOID MATRIX IN DECALCIFIED BONE
S. YOSHIKIT, . UENO,T . AKITA A ND M. YAMANOUCHI
Department of Oral Pathology, School of Dentishy, Showa University, Shinagawaku, Tokyo 142 Japan

ABSTRACT. Several improvements on the original method of Yoshiki and coworkers for histological
identification of osteoid matrix in decalcified bone are described in this report. The first, fixation of
bone with neutral buffered formalin, a popular and stable fixative, should produce better tissue
morphology and ensure easy handling in any laboratory. The second is a simple test for aged cyanuric
chloride. Aged reagents show poor or no solubility in methanol and have almost no effect on
differential staining of osteoid matrix. The third is an application of an organic acid solution in place
of neutral EDTA for bone decalcification. Reduced decalcification time with the acid results in rapid
preparation of bone sections. Neutral formalin fixation, immersion in the cyanuric chloride solution,
decalcification with an organic acid, and hematoxylin and eosin staining, all quite routine laboratory
procedures, yield high quality results for identification of osteoid matrix in bone sections.