[Histonet] RE: positive control desperately needed for IDH1 R132H antibody

[Jerry Ricks]

Hi Johanna,

I think it is going to be hard to find brain tissue that has already worked up for that antibody.  You probably have to work up the antibody yourself.


But if it is this antibody:   http://www.ncbi.nlm.nih.gov/pubmed/19798509

It ought to work on 70% of astrocytomas.

Abcam sells control slides but they are kind of pricey.

http://www.abcam.com/Brain-tumor-human-astrocytoma-grade-II-tissue-slides-ab4679.html


Jerry Ricks
Research Scientist
University of Washington
Department of Pathology





> From: PREISZNE <@t> mail.etsu.edu
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Mon, 1 Oct 2012 18:47:16 +0000
> Subject: [Histonet] positive control desperately needed for IDH1 R132H	antibody
> 
> Hi,
> 
> we need to validate our IDH1 R132H antibody on a known positive tissue. We are a small lab and so far we could not get hold of a proven positive specimen. We did not have any luck contacting the antibody's manufacturer either.
> Could someone name a source from where we could get some sections from a specimen that's proven positive?
> My direct email address is: preiszne at etsu dot edu .
> Thanks!
> 
> Johanna Preiszner
> HT(ASCP), Ph.D.
> ETSU/QCOM
> Dept of Pathology
> 
> 
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