[Histonet] Microtome knives

[Jack Ratliff]

I might also add that Delaware Diamond Knives (DDK) also sells and sharpens microtome knives!
Jack

Jack L RatliffRatliff Histology Consultants, LLC317-281-1975



> From: pruegg <@t> ihctech.net
> To: max_histo_00 <@t> yahoo.it; jkrupp <@t> deltacollege.edu; Histonet <@t> lists.utsouthwestern.edu
> Date: Sun, 18 Nov 2012 07:50:11 -0700
> Subject: RE: [Histonet] Microtome knives
> CC: 
> 
> There are knife sharpening services Sturkey and Dorn and Hart are two that
> come to mind.  You can also find refurbished disposable blade holders and
> buy disposable blades, the blade holder you get will determine if you use
> low profile or high profile blades.
> 
> Patsy Ruegg, HT(ASCP)QIHC
> Ruegg IHC Consulting, LLC
> 40864 Arkansas Ave
> Bennett, CO 80102
> Phone: 303-644-4538
> Fax: 720-859-4110
> pruegg <@t> ihctech.net
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Massimo
> Sent: Sunday, November 11, 2012 2:45 AM
> To: Jon Krupp; Histonet <@t> lists.utsouthwestern.edu
> Subject: Re: [Histonet] Microtome knives
> 
> I prefer to sharpen my microtome knives by myself by hand.
> I have a vintage Cambridge Rocking Microtome and despite its age it works
> very well.
> Sharpening is a time consuming for the first time, it's depends on the
> conditions of the blade edge.
> Once you have a nice cutting profile its maintenance it's quite easy and it
> takes a few minutes by
> stroking the knife on a flat glass with oil and a bit of aluminium oxide
> powder (3 -1 micron grits).
> For me sharpening and honing of a microtome knife has became a secondary
> "hobby".
> A solid knife has the advantage, compared to a disposable blade, to be
> liable to less vibrations.
> 
> Kind Regards,
> Massimo Tosi
> 
> 
> "A humble Chemical
> Engineer who loves Histology"
> 
> 
> 
> 
> 
> ________________________________
>  Da: Jon Krupp <jkrupp <@t> deltacollege.edu>
> A: Histonet <@t> lists.utsouthwestern.edu 
> Inviato: Venerdì 9 Novembre 2012 19:49
> Oggetto: [Histonet] Microtome knives
>  
> Greetings
> 
> I need some advice regarding microtome knives. I am not  histo tech, I did
> all my sectioning in a plant research lab, but now I find myself needing to
> learn more about histo type methods.
> 
> We have microtomes, AO 820's, and we have a bunch of donated knives. I need
> advice about whether it would be better to find a knife sharpener and use
> the microtome knives we have, or check into getting a disposable knife
> holder. 
> 
> When I was sectioning, we just used a simple razor blade holder. Now I see
> references to high profile and low profile blades and holders, and I don't
> know the difference. 
> 
> Anyone willing to help me out?
> 
> Thanks
> 
> Jon
> 
> Jonathan Krupp
> Delta College
> 5151 Pacific Ave.
> Box 212
> Stockton, CA  95207
> 209-954-5284
> jkrupp <@t> deltacollege.edu
> 
> Find us on Facebook @
> Electron Microscopy at SJ Delta College
> 
> 
> 
> 
> 
> 
> 
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