[Histonet] Ultra

[Daniel Hewitt]

We have the same issue from time to time on our Ultra, our vortex mixers
seem to clog after about 3-4 months of use. The engineer comes in,
unclogs them and everything is good for another 3-4 months. They have
never really been able to figure it out why they clog but, but
thankfully the service is still covered, just a large nuisance.

Daniel Hewitt
Histology Supervisor, HVS
412-749-7371

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-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bruce
Gapinski
Sent: Monday, November 26, 2012 1:50 PM
To: 'cthornton <@t> dahlchase.com'; 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Ultra

I too have had problems with my Ultra. I am so glad I place a control at
the top of every slide. Sometimes no Hematoxylin, sometimes no positive
staining. I've documented half a dozen cases.
                One thing we noticed is that the antibody vials get
plugged up from the protein in the antibody. We inspect every vial
before we place it on the instrument. The other thing we look for is
antibody in the spout of the vial. It will recede. Called Ventana, and
they said " There are plenty of extra drops in the vial, so prime the
vial. Bad advise, here's why. The instrument has no idea how many drops
are disposed of during priming. So we ended up with another bunch of
primaries with no drops left. Now we prime without expelling any reagent
and how that works better.
                With this problem I feel Ventana owes us URA (Ultimate
Reagent Access) then we can prime as we go.

Bruce Gapinsk HT (ASCP)
Chief Histologist
Marin Medical Laboratories
PathGroup SF


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