AW: [Histonet] Ultra users

[Gudrun Lang]

Clare,
last month we had recurring troubles with the Ultra.
With a couple of runs the slides were not stained correctly. In one run we
had slides with no colour, with dab-colour, with purple-hematoxylin color,
with specific antibody-staining and without. The whole panorama.
Some runs were completed without any alarm. Some stopped with the alarm
that's due to the fact, that the arm didn't find the right position at the
end of its turn. This error was told to be caused by an invalid sensor, that
is humpered by too much moisture (fog) in the ultra.

Ventana said the slides are the culprit (like every time). We took they
recommended from Leica. And had the same troubles.
After that no one really had an idea. Now we've got a new Ultra. Tomorrow I
know more, if it was the instrument or the reagens.

Our Ultra is now about two years old and has been running quite 24 hours a
day....
I also have the suspiscion, that the spinning arm and the hammer are the
cause. 

Please tell me more about your troubles. And how you fixed it.
Kind regards
Gudrun

-----Ursprüngliche Nachricht-----
Von: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] Im Auftrag von Clare
Thornton
Gesendet: Montag, 26. November 2012 15:42
An: histonet <@t> lists.utsouthwestern.edu
Betreff: [Histonet] Ultra users

Has anyone encountered incorrect/inappropriate staining using the Ultra?
Twice we have had slides that looked like they had the wrong antibody
applied, although the slides and dispensers were labeled correctly (once
with TTF-1, once with p16; both had membranous staining).  I was wondering
if where the reagent wheel both spins and moves on the arm, occasionally it
doesn't quite make it to the dispenser it is supposed to and applies the
wrong one.  Does anyone have any experience/ideas as to why this might
happen?

thank you!



Clare J. Thornton, HTL(ASCP)QIHC
Assistant Histology Supervisor
Dahl-Chase Diagnostic Services
417 State Street, Suite 540
Bangor, ME 04401
cthornton <@t> dahlchase.com


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