[Histonet] H&E Question

[Lynette Pavelich]

I know this is out of the box, but it happened to me so I thought I'd pass this along. We had the same issue with our chief pathologist.......hematoxylin was too light. We tried everything, but she still complained!! Then we diluted our Eosin Y 1:1 with 95% alcohol and then she was happy. Crazy, I know, but something to think about!

Best of luck, and please let us know your findings!
Lynette

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] on behalf of Goins, Tresa [TGoins <@t> mt.gov]
Sent: Wednesday, November 14, 2012 11:02 AM
To: Jaclyn Pitts; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] H&E Question

Jackie -

Try a different hematoxylin - we switched to Platinum Line Modified Harris Hematoxylin - we save money and the pathologists prefer the results.

Tresa

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts
Sent: Wednesday, November 14, 2012 8:51 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E Question

Hi All,
I am having a problem with my staining, ( well, I think its pretty good but the path says its not cark enough for her) So, I am using Azer Scientific Hematoxylin Extra and I initially started out with 2.5 min in it. I upped it to 5  min and then to 10 and 12 min. The problem is the control I have works and looks great at the 2.5 min. Besides just keeping it in the heme longer what else can I do? I have cut a few extra dummy slides today so I can try different things out with them. I am tired of hearing from the path everyday that the heme is too light for her.
I am a new lab for a derm clinic and I work alone so its basiclly up to me to figure this out. Please help! Thanks!

Jackie
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