[Histonet] H&E Question

[Craven Peter (NHS HIGHLAND)]

Jaclyn
it sounds daft but check the pH of your Eosin we found this can be strongly acidic.

Peter

Peter L Craven FIBMS
Pathology Department
Raigmore Hospital
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jaclyn Pitts [pitts.jaclyn <@t> gmail.com]
Sent: 14 November 2012 03:50 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] H&E Question

Hi All,
I am having a problem with my staining, ( well, I think its pretty good but
the path says its not cark enough for her)
So, I am using Azer Scientific Hematoxylin Extra and I initially started
out with 2.5 min in it. I upped it to 5  min and then to 10 and 12 min. The
problem is the control I have works and looks great at the 2.5 min. Besides
just keeping it in the heme longer what else can I do? I have cut a few
extra dummy slides today so I can try different things out with them. I am
tired of hearing from the path everyday that the heme is too light for her.
I am a new lab for a derm clinic and I work alone so its basiclly up to me
to figure this out. Please help! Thanks!

Jackie
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