[Histonet] Technovit 9100 new fails to polymerize

[Marsh, Nannette]

We had the same problems.  We switched to Immunobed and that is wonderful.  Sets up every single time.  Give it a try.  Best regards, Nanne 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Jean-Philippe Berteau
Sent: Wednesday, November 14, 2012 4:14 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Technovit 9100 new fails to polymerize

Hello, 

 

As I wrote you some weeks ago, we started to use Technovit 9100 New

for tissue embedding. You helped me to solve my previous problems.

And I hope, you will help me to solve my present problems. 

 

 

Sorry, I can explain my problem as follow :

The main problem is that we are having some troubles with the

polymerization of Technovit 9100 New. The polymerization mixture does

not want to become hard. We filled trial moulds with 3 ml of

polymerization mixture. Moulds were vacuumed and sealed. We tried to

perform the polymerization reaction at -30 degrees C, -20 degrees C,

-4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails

to polymerize. After a week, the polymerization mixture becomes

gelatinous but not hard. We used the manufacturer's protocol. The preparation of the polymerization mixture was precise (I accurately prepared stock solutions several times). I cannot understand where is the problem

May be it is necessary to add more activator (dibenzoyl peroxide and

N,N-3,5-tetramethylaniline).  May be it is necessary to mix stock solutions A and B in another

proportion (not 9:1).

Is it necessary to prepare the solution at 4 degrees too ?  

What should I do?  

 

Thank you in advance, 

 

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