[Histonet] Technovit 9100 new fails to polymerize
jean-philippe.berteau <@t> tu-harburg.de
Wed Nov 14 04:13:58 CST 2012
As I wrote you some weeks ago, we started to use Technovit 9100 New
for tissue embedding. You helped me to solve my previous problems.
And I hope, you will help me to solve my present problems.
Sorry, I can explain my problem as follow :
The main problem is that we are having some troubles with the
polymerization of Technovit 9100 New. The polymerization mixture does
not want to become hard. We filled trial moulds with 3 ml of
polymerization mixture. Moulds were vacuumed and sealed. We tried to
perform the polymerization reaction at -30 degrees C, -20 degrees C,
-4 degrees C, +4 degrees C, +20 degrees C. Technovit 9100 New fails
to polymerize. After a week, the polymerization mixture becomes
gelatinous but not hard. We used the manufacturer's protocol. The
preparation of the polymerization mixture was precise (I accurately prepared
stock solutions several times). I cannot understand where is the problem
May be it is necessary to add more activator (dibenzoyl peroxide and
N,N-3,5-tetramethylaniline). May be it is necessary to mix stock solutions
A and B in another
proportion (not 9:1).
Is it necessary to prepare the solution at 4 degrees too ?
What should I do?
Thank you in advance,
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