[Histonet] Problem with cardiomyocytes staining

[Amos Brooks]

Hi Laura,
     I do this stain very frequently. It can be finickey. You should make
very sure the pH is below 2.5. It could be that you have an old solution
and over time these tend to drift toward a neutral pH. This will affect the
staining. Sometimes the solutions need to be discarded and either re-made
or have new purchased.

Best of luck,
Amos

On Tue, Nov 13, 2012 at 1:00 PM,
<histonet-request <@t> lists.utsouthwestern.edu>wrote:

> Message: 18
> Date: Tue, 13 Nov 2012 18:32:09 +0100 (CET)
> From: "Laura Avogaro" <avogaro <@t> science.unitn.it>
> Subject: [Histonet] Problem with cardiomyocytes staining
> To: histonet <@t> lists.utsouthwestern.edu
> Message-ID:
>         <3064.192.168.178.78.1352827929.squirrel <@t> www.science.unitn.it>
> Content-Type: text/plain;charset=iso-8859-1
>
> Dear Histonetters,
> I am new in histology so I ask if someone have experience with cardiac
> tissue. Recently I performed a Picrosirius Red staining (pig atrium, 5
> microns thickness) but in some sections I noted cardiomyocytes colored
> brown (instead of pale yellow).I wonder if it is probably due to the
> thickness of the section or something alse(unfortunately I use an old
> microtome whose probably fails to work in the correct way).
> Thank you in advance
> Best regards
>
> Laura
>