[Histonet] Iron Stain

[Rene J Buesa]

I think that instead trying to guess that "mysterious" step, you should contact the company that developed the kit and ask them.
Maybe their reagents require that.
Never assume!
René J.


________________________________
From: "Parker, Helayne" <HParker <@t> Skaggs.Net>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Monday, November 12, 2012 2:31 PM
Subject: [Histonet] Iron Stain


Hi All,
  Is there any specific reason why one iron stain kit I have recommends that you air-dry the bone marrow and blood smears after the Fe stain is completed and then coverslip them while other kits don't mention that at all.  I have always stained my smears then dehydrate, clear and coverslip them like the tissue slides (at the end). Ergo Fe stain, rinse, paragon, rinse, 95% Alc, 100% Alc, xylene and coverslip.  

Helayne Parker, H.T. (ASCP)
Pathology Section Head

Skaggs Regional Medical Center
The Best Place to Get Better

P.O. Box 650, Branson Missouri 65615
Direct: 417-335-7254
Fax: 417-335-7127
E-Mail: hparker <@t> skaggs.net
Web: http://www.skaggs.net/

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