[Histonet] Mold Release Problems

Rene J Buesa rjbuesa <@t> yahoo.com
Sat Nov 10 09:37:03 CST 2012


Mold release usually contains isopropyl alcohol and if you use too much, the alcohol will "eat into the block" as you describe and that will cause the sections to explode. Everything  you have described.
If you want to use the mold release use less and better yet, do not use mold release (in reality should be called "block" release).
Anyway, if you cool the block properly you do not need to use this product because a cold block will contract becoming "smaller" than the mold and in consequence will be easier to be released.
René J.


________________________________
From: AFirst Name Gee <bluebird8081 <@t> yahoo.com>
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Friday, November 9, 2012 6:16 PM
Subject: [Histonet] Mold Release Problems

Does anyone know of a reference for the problems from using mold release?  When too much is used on the mold it eats into the block and causes the ribbons to explode on the water bath and creates an artifact in the tissue.I cannot find a reference for it or any published material about the problem but have been to seminars where it was discussed.When it is not used the problem never occurs.
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