[Histonet] Microtome knives

[Jon Krupp]

Greetings

I need some advice regarding microtome knives. I am not  histo tech, I did all my sectioning in a plant research lab, but now I find myself needing to learn more about histo type methods.

We have microtomes, AO 820's, and we have a bunch of donated knives. I need advice about whether it would be better to find a knife sharpener and use the microtome knives we have, or check into getting a disposable knife holder. 

When I was sectioning, we just used a simple razor blade holder. Now I see references to high profile and low profile blades and holders, and I don't know the difference. 

Anyone willing to help me out?

Thanks

Jon

Jonathan Krupp
Delta College
5151 Pacific Ave.
Box 212
Stockton, CA  95207
209-954-5284
jkrupp <@t> deltacollege.edu

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