[Histonet] decal affecting IHC

[Rene J Buesa]

IHC reactions can be affected by decalcification especially if:
1- the tissue is not perfectly fixed before going into decalcification
2- decalcification is done at temperature above room temp.
3- decal solution is too acid, specially made with inorganic acids.
If at all possible decalcification should be done with a chelating agent, like EDTA
René J.


________________________________
From: Diana McCaig <dmccaig <@t> ckha.on.ca>
To: "'histonet <@t> lists.utsouthwestern.edu'" <histonet <@t> lists.utsouthwestern.edu> 
Sent: Friday, November 9, 2012 12:34 PM
Subject: [Histonet] decal affecting IHC

Does decalcifying tissue  (Calex II) affect the antibody reaction for IHC in bony tissue?

Diana

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