[Histonet] Collagen fragmentation in skin sections after heat retrieval

[Elizabeth Chlipala]

For skin and friable samples we retreive at 70C for 2 hours, works for most antibodies, but not all.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308
(303) 682-3949 office
(303) 881-0763 cell
(303) 682-9060 fax
liz <@t> premierlab.com

Ship to address:

Premier Laboratory, LLC
1567 Skyway Drive, Unit E
Longmont, CO 80504
________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Sally Price [sprice2003 <@t> gmail.com]
Sent: Wednesday, November 07, 2012 6:46 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Collagen fragmentation in skin sections after heat  retrieval

Our lab has been experiencing a problem recently and we could use some
input on what might be causing it and what we might be able to do to
prevent it.  The issue is a very noticeable fragmentation of the dermal
collagen within most skin specimens, which isn't visible after H&E
staining, but is after heat-retrieval.  We think our retrieval is pretty
gentle: It's performed in a veggie steamer (approx. 92oC) for 40 min,
followed by 15 min colloing on the countertop before rinsing.  We've tried
a variety of charged slides to see if the collagen holds on better, but it
doesn't.  We think our processing is pretty routine as well.  If it will
help, I can send a photo of this phenomenon to anyone who's interested.
Thanks,
Sally
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