[Histonet] How to fix frozen section?

[Connolly, Brett M]

Amy,

We use ice-cold acetone/ethanol (3:1) for 10'...works for all of our antigens so far.  


Brett M. Connolly, Ph.D.
Principal Scientist, Imaging Dept.
Merck & Co., Inc.
PO Box 4, WP-44K
West Point, PA 19486
brett_connolly <@t> merck.com
T- 215-652-2501
F- 215-993-6803


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Lee
Sent: Tuesday, November 06, 2012 2:41 PM
To: histonet
Subject: [Histonet] How to fix frozen section?

Hello,
 
I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me?
 
 
Thanks,
 
Amy
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