[Histonet] How to fix frozen section?

Sebree Linda A LSebree <@t> uwhealth.org
Tue Nov 6 13:44:58 CST 2012

It depends upon what marker you're staining for.  We fix our renal biopsies for C4d in acetone for 10". 

Linda A. Sebree

University of Wisconsin Hospital & Clinics
IHC/ISH Laboratory
600 Highland Ave.
Madison, WI 53792

FAX: (608)262-7174

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Amy Lee
Sent: Tuesday, November 06, 2012 1:41 PM
To: histonet
Subject: [Histonet] How to fix frozen section?

I will receive some frozen tissue sections that are not fixed. I rarely do IHC on frozen sections. I searched some articles about fixation. Some use 50/50 of acetone/alcohol and some use only alcohol. Could you please recommend a fixation method for me?
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