[Histonet] Background staining on H pylori

Deloris Carter delsuec <@t> gmail.com
Mon Nov 5 17:15:56 CST 2012

I'm getting a lot of background staining on my HP's.  We use a Ventana
BenchmarkXT with ultraView DAB.  The problem seems to be escalating of
late, and I'm not sure why.  We use Hollande's on our GI biopsies, and run
them on a shorter run.  Nothing has changed in the processing of the
specimens, or the IHC procedure.  The antibody dispenser is a newer lot,
but not brand new, as we get the larger size due to the high volume of HP's
we run. Any ideas?

Deloris Carter HT(ASCP)

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