[Histonet] Snap frozen to FFPE

Andrea T. Hooper andreahooper <@t> rocketmail.com
Mon Nov 5 12:31:37 CST 2012


Hi All, Do you have a protocol for formalin fixation (and processing to FFPE) of a previously snap frozen piece of tissue? Or advice as to the best method of snap freezing for such a process (assume isopentane slurry)? We have done this for several tissues with our standard fixation and processing as we would for fresh wet tissue and have noticed an artifact. Advice is welcome.
 
Thank you, Andrea 


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