[Histonet] Elastic Stain

[Rena Fail]

Have you tried Resorcin fucshin H&E?
1       g RF
100 ml 70% ethanol
1      ml HCL

Stain in RF solution 15-30 minutes , according to depth desired
wash off excess stain in 95% alcohol
Wash in tap water 3-5 minutes
Follow with routine H&E

Rena Fail
On Thu, May 24, 2012 at 11:37 AM, Vickroy, Jim <Vickroy.Jim <@t> mhsil.com>wrote:

> We are still looking for an Elastic/H&E stain procedure for lung tissue.
> Please submit procedures to Jim Vickroy.   vickroy.jim <@t> mhsil.com<mailto:
> vickroy.jim <@t> mhsil.com>
>
> A few of you have responded with some suggestions but we still have not
> found the right procedure.   Currently we stain for elastic with Verhoeff's
> Elastic Stain and  use Van Gieson's stain  as a counterstain and the
> pathologist would like us to use a hematoxylin and eosin counterstain
> instead.  We have tried to use the H&E as a counterstain but it is still
> not where he wants it to be.   Does anybody have some experience with this
> that they would like to share?
>
> Thanks
>
>
> James Vickroy BS, HT(ASCP)
>
> Surgical  and Autopsy Pathology Technical Supervisor
> Memorial Medical Center
> 217-788-4046
>
>
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