[Histonet] disposal of 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB)

[Rene J Buesa]

I used to neutralize DAB with 0.2M potassium permanganate + 2.0 M sulfuric + decolorize with ascorbic acid, but the procedure is too cumbersome.
Others prefer to give the waste to a company that will dispose of it BUT remember that no matter who "disposes" of your wastes, YOU and NOT the disposing company are responsible is something happens,
The same happens with disposing of used formalin and xylene.
René J.

--- On Thu, 5/24/12, idimitro <@t> mun.ca <idimitro <@t> mun.ca> wrote:


From: idimitro <@t> mun.ca <idimitro <@t> mun.ca>
Subject: [Histonet] disposal of 3, 3'-Diaminobenzidine Tetrahydrochloride (DAB)
To: histonet <@t> lists.utsouthwestern.edu
Date: Thursday, May 24, 2012, 8:37 AM


Dear HistoNet Community,


Recently we purchased an immunostainer . In the waste from the immunostainer there are small quantities of 3,3'-Diaminobenzidine Tetrahydrochloride (DAB) in the amount of e 35-38 mg per 20L of waste.
I want to hear from the community how you dispose of it. Do you use any of the methods available (permanganate/sulphuric acid and hydrogen peroxide/HRPA) to convert DAB to non-mutagenic compounds?

Thank you,

Iliana Dimitrova, RT, B.Tech., M.Sc.

Histology Supervisor
Medical Education and Laboratory Support Services (MELSS)
Faculty of Medicine
Memorial University of Newfoundland
St. John's, NL Canada A1B 3V6





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