[Histonet] Antibody titer

[Richard Cartun]

Yes, I always want to see the immunoreactivity "drop-off" (or even disappear) with a higher dilution(s).  Otherwise, you might be wasting antibody by using a dilution that is too concentrated.  Also, be careful with the recommended dilution.  That dilution is only applicable if you are using the exact same staining conditions.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> Courtney Pierce <Courtney.Pierce <@t> quintiles.com> 5/23/2012 12:57 PM >>>
When you titer a new antibody you go one below and one above the recommend dilution. If you find that the one above the dilution works do you go then out two more to make sure they don't work? This is a question in our lab right now.

Courtney Pierce
IHC Specialist
Quintiles
Translational R&D - Oncology
Innovation
Navigating the new health

610 Oakmont Lane
Westmont, IL 60559

Office: + 630-203-6234
courtney.pierce <@t> quintiles.com 

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