[Histonet] Neutrophil Antibodies

[Jan Shivers]

Hi John,

In the past, I used Neutrophil Elastase from Dako on monkey FFPE tissue; no
pretreatment needed; dilution with lot used = 1:50.  Worked great.
Jan Shivers
UMN Vet Diag Lab
On Sat, May 5, 2012 at 9:59 AM, John Shelley <jshelley <@t> sanfordburnham.org>wrote:

> Hi All in Histoland,
>
> Problem solving never ends! I am working up two antibodies for neutrophils
> which are NIMP-R14 from Abcam which it is a rat monoclonal and also
> Anti-Neutrophil Elastase also from Abcam but this is a Rb polyclonal. I
> have tried different conditions without any luck and was wondering if
> anyone has worked with these antibodies either from Abcam or another
> supplier and might have working conditions that have given results in the
> past. We are trying this on FFPE sections which the antibodies say they
> work on but I am thinking that I may try frozens. I was trying to avoid
> this however because I am using these antibodies on mouse WAT. Any help
> would be appreciated. Thanks!!!
>
> Happy Monday morning!!!
> Kind Regards!
> John J Shelley
>
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>