[Histonet] how often do you change your hematoxylin?

[Jackie O'Connor]

Absolutely it becomes diluted from water - are you hand staining or auto?  Autostainers tend to allow a longer time for excess water to drip off - more consistency.  Are you covering your htox when not in use?  Do you notice any type of sheen on the surface prior to staining?  Have you noticed weaker staining after 1K slides?  
Jackie O'


-----Original Message-----
From: Meryl Roberts <meryl50 <@t> hotmail.com>
To: histonet <histonet <@t> lists.utsouthwestern.edu>
Sent: Sun, May 6, 2012 3:51 pm
Subject: [Histonet] how often do you change your hematoxylin?





We are currently using a vintage solution, and the manufacturer states that it 
should be good for 2500 slides. We are not a high volume lab; and currently I am 
finding that the longest I can stretch it out for is 2 weeks max, in which time 
we have stained less than 1000 slides. How often does on average do you change 
your hematoxylin? Do you think maybe it could be getting diluted by water 
dripping from the slides; or maybe it is oxidising in the air? Thanks, Meryl 		 	   		  
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