[Histonet] .._Bone Marrows

[Rene J Buesa]

Our bone marrow trephines were fixed with neutral buffered formalin from the moment they were taken to about 6 PM (from 8 to 12 hours) in NBF before placing them in EDTA overnight.
The only thing is that we were absolutely sure that the pH of the NBF was 7.0 Always got very good results.
René J.

--- On Thu, 5/3/12, Hannen, Valerie <Valerie.Hannen <@t> parrishmed.com> wrote:


From: Hannen, Valerie <Valerie.Hannen <@t> parrishmed.com>
Subject: [Histonet] .._Bone Marrows
To: "histonet <@t> lists.utsouthwestern.edu" <histonet <@t> lists.utsouthwestern.edu>
Date: Thursday, May 3, 2012, 11:26 AM



Good morning Histonetters!!!

Our Pathologist has been reading again !!  He read an article about Bone Marrow fixation. I have a few questions to pose to you all.

1) Do you typically fix your Bone Marrow biopsies overnight before decalcification?  We currently do not.

2) What are you fixing them in?

Formalin?        Zinc Formalin? (this is what we currently are using)       B Plus? (this was the fixative mentioned in the article).


Any and all input will be greatly appreciated!!


Valerie

Valerie A. Hannen, MLT(ASCP),HTL,SU(FL)
Histology Section Chief
Parrish Medical Center
951 N. Washington Ave.
Titusville, Florida 32976
Phone:(321) 268-6333 ext. 7506
Fax: (321) 268-6149
valerie.hannen <@t> parrishmed.com




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