[Histonet] Tissue Micro Array Devices

Freeman, Carol Carol.Freeman <@t> utoledo.edu
Fri Mar 23 08:37:36 CDT 2012 

 
Good Morning and Happy Friday fellow histonetters :)

I am looking into getting a Tissue Micro Array Device to make some nice multi-tissue (normal) and multi-tumor blocks for controls, validations and lot checks for IHC.  Has anyone any advice for purchasing one, any favorites or any we should stay from....Any responses are appreciated.

Thanks in advance ;)
Carol Freeman

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histonet-request <@t> lists.utsouthwestern.edu
Sent: Friday, March 23, 2012 8:46 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: Histonet Digest, Vol 100, Issue 32

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Today's Topics:

   1. RE:  Nocardia sp in FFPE (gayle callis)
   2. Subject: [Histonet] Paraffin Block Shipping Question
      (White, Lisa M.)
   3. RE: re: April fools prank (Podawiltz, Thomas)
   4. Fungus control (Cynthia Robinson)
   5. softening cartilage (Gerjets, Diane M [V PTH])
   6. Re: Fungus control ( thernandez709 <@t> yahoo.com )
   7. Retention Standards (histotech <@t> imagesbyhopper.com)
   8. RE: re: April fools prank (O'Donnell, Bill)
   9. RE: Retention Standards (Bernice Frederick)
  10. RE: softening cartilage (Elizabeth Chlipala)
  11. RE: Retention Standards (histotech <@t> imagesbyhopper.com)
  12. RE: Retention Standards (histotech <@t> imagesbyhopper.com)
  13. Re: Retention Standards (Rich Yeo)
  14. RE: Retention Standards (histotech <@t> imagesbyhopper.com)
  15. Re: Fixatives for breast (Richard Cartun)
  16. Re: Nocardia procedure (Tony Reilly)
  17. Part Time Histotech with Benefits near Portland, OR
      (Katelin Lester)
  18. Post question (marc centofante)
  19. Problems using tomato lectin (James S.)
  20. Mouse abs on mouse tissue method (James S.)
  21. PROTOCOL FOR COLOR CODING BIOPSY CASSETTES
      (Bernadette del Rosario)


----------------------------------------------------------------------

Message: 1
Date: Thu, 22 Mar 2012 11:34:14 -0600
From: "gayle callis" <gayle.callis <@t> bresnan.net>
Subject: [Histonet] RE:  Nocardia sp in FFPE
To: "Histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <001101cd0852$01a196c0$04e4c440$@bresnan.net>
Content-Type: text/plain;	charset="us-ascii"

Jeanine, 

 

I always recall what the third edition AFIP manual (green) had to say about
staining Nocardia successfully.   Unfortunately, I didn't see an antibody
for Nocardia.  

 

Several stains work and maybe have to do a combination of stains correlating
results using adjacent sections?   

 

First choice would be GMS but you have to do some extra times in silver
solution according to AFIP manual/Luna.   In order to get complete
development of filaments if different types/strains are suspected, run two
slides with one at 60 minutes and the other at 90 minutes in the silver
staining solution.   Be sure to use chromic acid as the oxidizer.
Churukian Shenk had a modified GMS that is supposed to work too, but nothing
was said about extended times in methenamine silver solution.       

 

Fite's 

 

Rojas-Darby-Nochomovitz Method for Norcardia, TB and Leprae.  This is
similar to Fites but uses a Ziehl Neelsen carbol fuchsin modified with
mineral oil  (from  Luna's Histopathological Methods and Color Atlas book)

 

Luna Parker Method for Nocardia Asteroides  (Luna book) with filaments
appearing red.  

 

Brown and Brenn should show the organism too. 

 

Good luck

 

Gayle M. Callis

HTL/HT/MT(ASCP)

 

 

 

You Wrote: 

 

I need the absolute best, most fool-proof special stain protocol for the
staining of Nocardia in FFPE tissue.

 

Thanks in advance!

 

Jeanine H. Bartlett, BS HT(ASCP), QIHC

Centers for Disease Control and Prevention

Infectious Diseases Pathology Branch

1600 Clifton Road, NE

MS/G-32

Atlanta, Ga 30333

404-639-3590

Jeanine.bartlett <@t> cdc.hhs.gov



------------------------------

Message: 2
Date: Thu, 22 Mar 2012 14:15:36 -0400
From: "White, Lisa M." <Lisa.White3 <@t> va.gov>
Subject: Subject: [Histonet] Paraffin Block Shipping Question
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<2B2ECF33934F5D4996D8BE03EFDF3976097677AD <@t> VHAV09MSGA3.v09.med.va.gov>
Content-Type: text/plain; charset="us-ascii"

 

EXAKT Technologies:  Cold D-Pak for transport stock# MD8702V06; Frozen
d-Pak for transport stock # MD8703V06

 

With current regulations on labeling and shipping of diagnostic material
if you need to ship on ice or dry ice the above company has pre-labeled
containers with all the federal requirements, etc as well as all the
required packaging for the specimen and documents.  We have to take
training every year and it was explained to us that if something leaks
all the powers that be out there go after the person who has their name
on the shipping label (also whoever packs it has to be the one to do the
label with their name).  Blocks with a cold pack is a good idea during
warm days.  After such a mild winter I bet summer will be interesting.

 

Also recently I was trying to find something to put a block in to mail
and I had been putting them in a side loaded 5 slide container but could
only get one in each one (but they cost $$$).  I had been coverslipping
and looked down at the empty coverslip container, VIOLA!  Take the
packaging out and you can get 2-3 blocks in there and they won't get
squashed in the shipment and the container is free.

 

Lisa White, HT(ASCP)

Supervisory HT

James H. Quillen VAMC

PO Box 4000

Corner of Veterans Way and Lamont

PLMS 113

Mountain Home, TN 37684

423-979-3567

423-979-3401 fax

 



------------------------------

Message: 3
Date: Thu, 22 Mar 2012 14:20:26 -0400
From: "Podawiltz, Thomas" <tpodawiltz <@t> lrgh.org>
Subject: RE: [Histonet] re: April fools prank
To: Kara Lee <karabou76 <@t> hotmail.com>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<38667E7FB77ECD4E91BFAEB8D986386324FB7E26B7 <@t> LRGHEXVS1.practice.lrgh.org>
	
Content-Type: text/plain; charset="us-ascii"

I used to be real bad about pulling pranks in my younger days. If fact, there were three of use in our Navy lab that pranked each other all the time. Then to the horror of the rest of the staff we joined forces and starting pranking everyone else. It finally got to the point that all we had to do was make a comment about something and watch everyone's paranoia go up. Funny thing was on April first we never did a thing...and that drove the staff nuts. 



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kara Lee
Sent: Thursday, March 22, 2012 11:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] re: april fools prank




One must always consider who the prank is being pulled on.  If the person is sensitive or not quite in line with your own "Humor" a prank should not be pulled.  As people working in the science industry, we have all been trained to observe.  If a person is not the type to deal well with that type of humor, don't bother them and let them be.  Or, ask if they want to be included in playing a prank on someone else who lives for seeing what their co-workers are doing to them next..I had a co-worker who was bummed when we were all too busy to mess with each others stuff one April 1st one year. 

Pranks and jokes should always make the other person laugh or smile just as much as you do, and don't forget to help them clean up the mess you made...but I think we all know that, right? ;)

Enjoy life with your co-worker friends, and laugh! I mean, we do spend over 50% of our lives with these people.

Cheers everyone!
Kara 


> From: SLB <@t> stowers.org
> To: hmarlatt26 <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> Date: Thu, 22 Mar 2012 07:14:41 -0500
> Subject: RE: [Histonet] re: april fools prank
> CC: 
> 
> Heather, I see no harm in a fake spider.  That would be fun.  But, I totally agree with what Barry just wrote.  Some of these other pranks are out of line, in my opinion.  I'm all for having fun and enjoying the spirit of the day, but I don't believe in aggravating or embarrassing someone for your satisfaction.  I have also pulled the hot dog trick on a resident pathologist.  He loved it. 
> Sharon
> 
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of heather marlatt
> Sent: Wednesday, March 21, 2012 10:25 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] re: april fools prank
> 
> I've been known to leave a fake spider in an embedder for the morning person.....although it wasn't april fools just for fun it got a great reaction :) _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 		 	   		  _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
THIS MESSAGE IS CONFIDENTIAL.  
This e-mail message and any attachments are proprietary and confidential information intended only for the use of the recipient(s) named above. If you are not the intended recipient, you may not print,distribute, or copy this message or any attachments.  If you have received this communication in error, please notify the sender by return e-mail and delete this message and any attachments from your computer. Any views or opinions expressed are solely those of the author and do not necessarily represent those of LRGHealthcare.




------------------------------

Message: 4
Date: Thu, 22 Mar 2012 14:24:09 -0400
From: "Cynthia Robinson" <robinsoc <@t> mercyhealth.com>
Subject: [Histonet] Fungus control
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4F6B27F9020000AF000082CC <@t> nodcdmg2.no.trinity-health.org>
Content-Type: text/plain; charset=US-ASCII

All,

We have a lung case that is loaded with fungus. Is anyone in need?



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc <@t> mercyhealth.com





------------------------------

Message: 5
Date: Thu, 22 Mar 2012 18:31:50 +0000
From: "Gerjets, Diane M [V PTH]" <dmnelson <@t> iastate.edu>
Subject: [Histonet] softening cartilage
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<56A357DCB599A04EAFC9C954B0A33DC20159DE39 <@t> ITSDAG2D.its.iastate.edu>
Content-Type: text/plain; charset="us-ascii"

I'm sorry if this topic has already been discussed, I don't get on the histonet  very often. Our lab will be getting a research project of horse cartilage. We don't have a good protocol for softening cartilage. We would appreciate any suggestions that any one might have.
Thank you so much!
Diane


------------------------------

Message: 6
Date: Thu, 22 Mar 2012 13:32:52 -0500
From: " thernandez709 <@t> yahoo.com " <thernandez709 <@t> yahoo.com>
Subject: Re: [Histonet] Fungus control
To: robinsoc <@t> mercyhealth.com,"histonet"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <860854.13965.bm <@t> smtp110.mail.bf1.yahoo.com>
Content-Type: text/plain;	charset=utf-8

Yes,we could definitely use some fungus. How can we arrange to get it ?

Sent from my HTC smartphone on the Now Network from Sprint!

----- Reply message -----
From: "Cynthia Robinson" <robinsoc <@t> mercyhealth.com>
To: "histonet" <histonet <@t> lists.utsouthwestern.edu>
Subject: [Histonet] Fungus control
Date: Thu, Mar 22, 2012 1:24 pm


All,

We have a lung case that is loaded with fungus. Is anyone in need?



Cindi Robinson HT(ASCP)
Mercy Medical Center
Dunes Medical Laboratories
350 W Anchor Dr
Dakota Dunes SD 57049
phone-712-279-2768
robinsoc <@t> mercyhealth.com



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 7
Date: Thu, 22 Mar 2012 14:37:38 -0400
From: <histotech <@t> imagesbyhopper.com>
Subject: [Histonet] Retention Standards
To: <histonet <@t> lists.utsouthwestern.edu>
Message-ID: <008601cd085a$deabc3d0$9c034b70$@imagesbyhopper.com>
Content-Type: text/plain; charset=US-ASCII

Does anyone have specific web links for CLIA, CAP, JC and, more specifically
for me, Florida specimen retention guidelines?  I want to update my
retention policy, but I want to ensure that I am using the strictest of the
guidelines.   I am looking for guidelines on retention of service
worksheets, specimen worksheets, requisitions etc and all things paper as
well.

THANKS for any help and direction you can provide,
Michelle




------------------------------

Message: 8
Date: Thu, 22 Mar 2012 12:40:19 -0600
From: "O'Donnell, Bill" <billodonnell <@t> catholichealth.net>
Subject: RE: [Histonet] re: April fools prank
To: "Podawiltz, Thomas" <tpodawiltz <@t> lrgh.org>,	"Kara Lee"
	<karabou76 <@t> hotmail.com>, <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<4940DF6D1C5FDF48931B6966AAEF93954D54D4 <@t> chimsx08.CHI.catholichealth.net>
	
Content-Type: text/plain; charset="ISO-8859-1"

Tom,

Not always a simple, fun prank, but bordering on the, I don't
know......psycopathic? (I won't mention them, as someone might actually
do them, then I'd have all those court appearences to deal with and
phycological evaluations.... I just don't have that kind of time
anymore) Of all the stunts we pulled, the ONLY one that ever got me in
trouble was putting carbonated water in someones waterbath. Certainly
one of the more benign efforts. I will likely do a lot of time in
purgatory for some of those, mostly because they still cause me to
smile, even after all these years. And to think we have become respected
professionals and pillars of society. Who'd-a-thunk it?  - Bill

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
Podawiltz, Thomas
Sent: Thursday, March 22, 2012 1:20 PM
To: Kara Lee; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] re: April fools prank

I used to be real bad about pulling pranks in my younger days. If fact,
there were three of use in our Navy lab that pranked each other all the
time. Then to the horror of the rest of the staff we joined forces and
starting pranking everyone else. It finally got to the point that all we
had to do was make a comment about something and watch everyone's
paranoia go up. Funny thing was on April first we never did a
thing...and that drove the staff nuts. 



Tom Podawiltz HT (ASCP)
Histology Section Head/Laboratory Safety Officer. 
LRGHealthcare
Laconia, NH 03246
603-524-3211 ext: 3220



-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Kara Lee
Sent: Thursday, March 22, 2012 11:59 AM
To: histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] re: april fools prank




One must always consider who the prank is being pulled on.  If the
person is sensitive or not quite in line with your own "Humor" a prank
should not be pulled.  As people working in the science industry, we
have all been trained to observe.  If a person is not the type to deal
well with that type of humor, don't bother them and let them be.  Or,
ask if they want to be included in playing a prank on someone else who
lives for seeing what their co-workers are doing to them next..I had a
co-worker who was bummed when we were all too busy to mess with each
others stuff one April 1st one year. 

Pranks and jokes should always make the other person laugh or smile just
as much as you do, and don't forget to help them clean up the mess you
made...but I think we all know that, right? ;)

Enjoy life with your co-worker friends, and laugh! I mean, we do spend
over 50% of our lives with these people.

Cheers everyone!
Kara 


> From: SLB <@t> stowers.org
> To: hmarlatt26 <@t> gmail.com; histonet <@t> lists.utsouthwestern.edu
> Date: Thu, 22 Mar 2012 07:14:41 -0500
> Subject: RE: [Histonet] re: april fools prank
> CC: 
> 
> Heather, I see no harm in a fake spider.  That would be fun.  But, I
totally agree with what Barry just wrote.  Some of these other pranks
are out of line, in my opinion.  I'm all for having fun and enjoying the
spirit of the day, but I don't believe in aggravating or embarrassing
someone for your satisfaction.  I have also pulled the hot dog trick on
a resident pathologist.  He loved it. 
> Sharon
> 
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu 
> [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of 
> heather marlatt
> Sent: Wednesday, March 21, 2012 10:25 PM
> To: histonet <@t> lists.utsouthwestern.edu
> Subject: [Histonet] re: april fools prank
> 
> I've been known to leave a fake spider in an embedder for the morning 
> person.....although it wasn't april fools just for fun it got a great 
> reaction :) _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> _______________________________________________
> Histonet mailing list
> Histonet <@t> lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
 
_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
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communication in error, please notify the sender by return e-mail and
delete this message and any attachments from your computer. Any views or
opinions expressed are solely those of the author and do not necessarily
represent those of LRGHealthcare.


_______________________________________________
Histonet mailing list
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http://lists.utsouthwestern.edu/mailman/listinfo/histonet

This electronic mail and any attached documents are intended solely for the named addressee(s) and contain confidential information. If you are not an addressee, or responsible for delivering this email to an addressee, you have received this email in error and are notified that reading, copying, or disclosing this email is prohibited. If you received this email in error, immediately reply to the sender and delete the message completely from your computer system.



------------------------------

Message: 9
Date: Thu, 22 Mar 2012 18:43:57 +0000
From: Bernice Frederick <b-frederick <@t> northwestern.edu>
Subject: RE: [Histonet] Retention Standards
To: "histotech <@t> imagesbyhopper.com" <histotech <@t> imagesbyhopper.com>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<62C639732D3F274DACED033EBDF6ADAF1E227469 <@t> CHCSPMBX4.ads.northwestern.edu>
	
Content-Type: text/plain; charset="us-ascii"

You can find the CAP retention policies on their web site
http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fportlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionForm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsletter.html&_state=maximized&_pageLabel=cntvwr


Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of histotech <@t> imagesbyhopper.com
Sent: Thursday, March 22, 2012 1:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Retention Standards

Does anyone have specific web links for CLIA, CAP, JC and, more specifically for me, Florida specimen retention guidelines?  I want to update my retention policy, but I want to ensure that I am using the strictest of the
guidelines.   I am looking for guidelines on retention of service
worksheets, specimen worksheets, requisitions etc and all things paper as well.

THANKS for any help and direction you can provide, Michelle


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 10
Date: Thu, 22 Mar 2012 12:46:41 -0600
From: Elizabeth Chlipala <liz <@t> premierlab.com>
Subject: [Histonet] RE: softening cartilage
To: "'Gerjets, Diane M [V PTH]'" <dmnelson <@t> iastate.edu>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<14E2C6176416974295479C64A11CB9AE011390CC5A09 <@t> SBS2K8.premierlab.local>
Content-Type: text/plain; charset="us-ascii"

Diane

You should not need to soften cartilage, we section cartilage from many species without any additional steps to routine processing.

Liz

Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC
Manager
Premier Laboratory, LLC
PO Box 18592
Boulder, CO 80308-1592
(303) 682-3949 office
(303) 682-9060 fax
(303) 881-0763 cell
www.premierlab.com

Ship to address:

1567 Skyway Drive, Unit E
Longmont, CO 80504


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Gerjets, Diane M [V PTH]
Sent: Thursday, March 22, 2012 12:32 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] softening cartilage


I'm sorry if this topic has already been discussed, I don't get on the histonet  very often. Our lab will be getting a research project of horse cartilage. We don't have a good protocol for softening cartilage. We would appreciate any suggestions that any one might have. Thank you so much! Diane _______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu http://lists.utsouthwestern.edu/mailman/listinfo/histonet



------------------------------

Message: 11
Date: Thu, 22 Mar 2012 14:46:36 -0400
From: <histotech <@t> imagesbyhopper.com>
Subject: RE: [Histonet] Retention Standards
To: "'Bernice Frederick'" <b-frederick <@t> northwestern.edu>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <008a01cd085c$1f945280$5ebcf780$@imagesbyhopper.com>
Content-Type: text/plain; charset=US-ASCII

Bernice, 
Thanks for that one!  That's one down.  :o)

JC anyone?

Florida??

:)

Michelle

-----Original Message-----
From: Bernice Frederick [mailto:b-frederick <@t> northwestern.edu] 
Sent: Thursday, March 22, 2012 2:44 PM
To: histotech <@t> imagesbyhopper.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Retention Standards

You can find the CAP retention policies on their web site
http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp
ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo
rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle
tter.html&_state=maximized&_pageLabel=cntvwr


Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Thursday, March 22, 2012 1:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Retention Standards

Does anyone have specific web links for CLIA, CAP, JC and, more specifically
for me, Florida specimen retention guidelines?  I want to update my
retention policy, but I want to ensure that I am using the strictest of the
guidelines.   I am looking for guidelines on retention of service
worksheets, specimen worksheets, requisitions etc and all things paper as
well.

THANKS for any help and direction you can provide, Michelle


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



-----
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Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12





------------------------------

Message: 12
Date: Thu, 22 Mar 2012 15:22:48 -0400
From: <histotech <@t> imagesbyhopper.com>
Subject: RE: [Histonet] Retention Standards
To: "'Bernice Frederick'" <b-frederick <@t> northwestern.edu>,
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <009401cd0861$2e0f3190$8a2d94b0$@imagesbyhopper.com>
Content-Type: text/plain; charset=US-ASCII

Here is an updated CAP compendium retention guidelines list.  This was last
updated in March, 2010

http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp
ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo
rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa
bel=cntvwr

Michelle

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Thursday, March 22, 2012 2:47 PM
To: 'Bernice Frederick'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Retention Standards

Bernice,
Thanks for that one!  That's one down.  :o)

JC anyone?

Florida??

:)

Michelle

-----Original Message-----
From: Bernice Frederick [mailto:b-frederick <@t> northwestern.edu]
Sent: Thursday, March 22, 2012 2:44 PM
To: histotech <@t> imagesbyhopper.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Retention Standards

You can find the CAP retention policies on their web site
http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp
ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo
rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle
tter.html&_state=maximized&_pageLabel=cntvwr


Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Thursday, March 22, 2012 1:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Retention Standards

Does anyone have specific web links for CLIA, CAP, JC and, more specifically
for me, Florida specimen retention guidelines?  I want to update my
retention policy, but I want to ensure that I am using the strictest of the
guidelines.   I am looking for guidelines on retention of service
worksheets, specimen worksheets, requisitions etc and all things paper as
well.

THANKS for any help and direction you can provide, Michelle


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



-----
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Checked by AVG - www.avg.com
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_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



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------------------------------

Message: 13
Date: Thu, 22 Mar 2012 12:35:49 -0700 (PDT)
From: Rich Yeo <histocontrols <@t> yahoo.com>
Subject: Re: [Histonet] Retention Standards
To: "histotech <@t> imagesbyhopper.com" <histotech <@t> imagesbyhopper.com>,
	'Bernice Frederick' <b-frederick <@t> northwestern.edu>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1332444949.74857.YahooMailNeo <@t> web140417.mail.bf1.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

There is another update from Jan. 2012. It should be on thier web site



R


________________________________
 From: "histotech <@t> imagesbyhopper.com" <histotech <@t> imagesbyhopper.com>
To: 'Bernice Frederick' <b-frederick <@t> northwestern.edu>; histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, March 22, 2012 3:22 PM
Subject: RE: [Histonet] Retention Standards
 
Here is an updated CAP compendium retention guidelines list.  This was last
updated in March, 2010

http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp
ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo
rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa
bel=cntvwr

Michelle

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Thursday, March 22, 2012 2:47 PM
To: 'Bernice Frederick'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Retention Standards

Bernice,
Thanks for that one!  That's one down.  :o)

JC anyone?

Florida??

:)

Michelle

-----Original Message-----
From: Bernice Frederick [mailto:b-frederick <@t> northwestern.edu]
Sent: Thursday, March 22, 2012 2:44 PM
To: histotech <@t> imagesbyhopper.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Retention Standards

You can find the CAP retention policies on their web site
http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2Fp
ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo
rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle
tter.html&_state=maximized&_pageLabel=cntvwr


Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Thursday, March 22, 2012 1:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Retention Standards

Does anyone have specific web links for CLIA, CAP, JC and, more specifically
for me, Florida specimen retention guidelines?  I want to update my
retention policy, but I want to ensure that I am using the strictest of the
guidelines.   I am looking for guidelines on retention of service
worksheets, specimen worksheets, requisitions etc and all things paper as
well.

THANKS for any help and direction you can provide, Michelle


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



-----
No virus found in this message.
Checked by AVG - www.avg.com
Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12



_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet



-----
No virus found in this message.
Checked by AVG - www.avg.com
Version: 2012.0.1913 / Virus Database: 2114/4886 - Release Date: 03/22/12


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

Message: 14
Date: Thu, 22 Mar 2012 16:50:24 -0400
From: <histotech <@t> imagesbyhopper.com>
Subject: RE: [Histonet] Retention Standards
To: "'Rich Yeo'" <histocontrols <@t> yahoo.com>,	"'Bernice Frederick'"
	<b-frederick <@t> northwestern.edu>,	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <000c01cd086d$6bac16b0$43044410$@imagesbyhopper.com>
Content-Type: text/plain;	charset="us-ascii"

I just got off the phone with CAP and the 2010 revision is their most
recent.  :o)

 

I also found the CLIA retention guidelines. they can be found at the
following URL:

https://www.cms.gov/CLIA/03_Interpretive_Guidelines_for_Laboratories.asp

 

2 down, 2 to go!

 

Michelle

 

From: Rich Yeo [mailto:histocontrols <@t> yahoo.com] 
Sent: Thursday, March 22, 2012 3:36 PM
To: histotech <@t> imagesbyhopper.com; 'Bernice Frederick';
histonet <@t> lists.utsouthwestern.edu
Subject: Re: [Histonet] Retention Standards

 

There is another update from Jan. 2012. It should be on thier web site

 

 

 

R

 

  _____  

From: "histotech <@t> imagesbyhopper.com" <histotech <@t> imagesbyhopper.com>
To: 'Bernice Frederick' <b-frederick <@t> northwestern.edu>;
histonet <@t> lists.utsouthwestern.edu 
Sent: Thursday, March 22, 2012 3:22 PM
Subject: RE: [Histonet] Retention Standards


Here is an updated CAP compendium retention guidelines list.  This was last
updated in March, 2010

http://www.cap.org/apps/cap.portal?_nfpb=true
<http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2F
p> &cntvwrPtlt_actionOverride=%2Fp
ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo
rm.contentReference%7D=policies%2Fpolicy_appPP.html&_state=maximized&_pageLa
bel=cntvwr

Michelle

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Thursday, March 22, 2012 2:47 PM
To: 'Bernice Frederick'; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Retention Standards

Bernice,
Thanks for that one!  That's one down.  :o)

JC anyone?

Florida??

:)

Michelle

-----Original Message-----
From: Bernice Frederick [mailto:b-frederick <@t> northwestern.edu]
Sent: Thursday, March 22, 2012 2:44 PM
To: histotech <@t> imagesbyhopper.com; histonet <@t> lists.utsouthwestern.edu
Subject: RE: [Histonet] Retention Standards

You can find the CAP retention policies on their web site
http://www.cap.org/apps/cap.portal?_nfpb=true
<http://www.cap.org/apps/cap.portal?_nfpb=true&cntvwrPtlt_actionOverride=%2F
p> &cntvwrPtlt_actionOverride=%2Fp
ortlets%2FcontentViewer%2Fshow&_windowLabel=cntvwrPtlt&cntvwrPtlt%7BactionFo
rm.contentReference%7D=laboratory_accreditation%2Fnewsletters%2F0301_2newsle
tter.html&_state=maximized&_pageLabel=cntvwr


Bernice Frederick HTL (ASCP)
Senior Research Tech
Pathology Core Facility
ECOGPCO-RL
Robert. H. Lurie Cancer Center
Northwestern University
710 N Fairbanks Court
Olson 8-421
Chicago,IL 60611
312-503-3723
b-frederick <@t> northwestern.edu

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of
histotech <@t> imagesbyhopper.com
Sent: Thursday, March 22, 2012 1:38 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Retention Standards

Does anyone have specific web links for CLIA, CAP, JC and, more specifically
for me, Florida specimen retention guidelines?  I want to update my
retention policy, but I want to ensure that I am using the strictest of the
guidelines.  I am looking for guidelines on retention of service
worksheets, specimen worksheets, requisitions etc and all things paper as
well.

THANKS for any help and direction you can provide, Michelle


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




 



------------------------------

Message: 15
Date: Thu, 22 Mar 2012 17:09:52 -0400
From: "Richard Cartun" <Rcartun <@t> harthosp.org>
Subject: Re: [Histonet] Fixatives for breast
To: "LaSalette Charette" <las.charette <@t> comcast.net>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4F6B5CE0.7400.0077.1 <@t> harthosp.org>
Content-Type: text/plain; charset=US-ASCII

1.) Formalin, 2.) Formalin, and 3.) Formalin.

Richard

Richard W. Cartun, MS, PhD
Director, Histology & Immunopathology
Director, Biospecimen Collection Programs
Assistant Director, Anatomic Pathology
Hartford Hospital
80 Seymour Street
Hartford, CT  06102
(860) 545-1596 Office
(860) 545-2204 Fax


>>> LaSalette Charette <las.charette <@t> comcast.net> 3/21/2012 12:59 PM >>>
What are the best fixatives for breast specimens? Also what the most commonly used fixatives out there?

LaSalette


_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet




------------------------------

Message: 16
Date: Fri, 23 Mar 2012 09:23:54 +1000
From: "Tony Reilly" <Tony_Reilly <@t> health.qld.gov.au>
Subject: Re: [Histonet] Nocardia procedure
To: "Jeanine (CDC/OID/NCEZID) Bartlett" <jqb7 <@t> cdc.gov>,
	"histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID: <4F6C4129.411C.0039.0 <@t> health.qld.gov.au>
Content-Type: text/plain; charset="us-ascii"

Hi Jeanine
 
I have always just used a Fite and it has worked well.  If you use a Gram you will find that they are Gram variable in their staining.
 
regards
Tony
 
 
 

Tony Reilly  B.App.Sc. , M.Sc.
Chief Scientist, Anatomical Pathology
Pathology Queensland-PA Laboratory
_________________________________________________
Clinical and Statewide Services Division| QueenslandHealth
 
Level 1, Building 15,Princess Alexandra Hospital
Ipswich Road,WOOLLOONGABBA  Qld4102
Ph: 07 3176 2412
Mob: 0402 139411
Fax: 07 3176 2930
Email: tony_reilly <@t> health.qld.gov.au
Web:  www.health.qld.gov.au/qhcss/
 
 


>>> "Bartlett, Jeanine (CDC/OID/NCEZID)" <jqb7 <@t> cdc.gov> 3/23/2012 1:54 am >>>
Hi all,

I need the absolute best, most fool-proof special stain protocol for the staining of Nocardia in FFPE tissue.

Thanks in advance!

Jeanine H. Bartlett, BS HT(ASCP), QIHC
Centers for Disease Control and Prevention
Infectious Diseases Pathology Branch
1600 Clifton Road, NE
MS/G-32
Atlanta, Ga 30333
404-639-3590
Jeanine.bartlett <@t> cdc.hhs.gov 

_______________________________________________
Histonet mailing list
Histonet <@t> lists.utsouthwestern.edu 
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

Message: 17
Date: Thu, 22 Mar 2012 16:42:49 -0700
From: Katelin Lester <katelin09htl <@t> gmail.com>
Subject: [Histonet] Part Time Histotech with Benefits near Portland,
	OR
To: Histonet <histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<CAOEzYbSMR6uxU2S0WpT8gGn5P+qNRbb9sczzQMZWdruwdNKzzQ <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

Part time certified HT or HTL wanted for busy GI practice in Tualatin,
Oregon.

Candidate must be ASCP certified and meet CLIA requirements to perform
gross dissection.

GI experience preferred.

This is a M-F position with no weekends , holidays or call.

Includes Full Medical & Dental benefits plus a 401K program.

Interested applicants should forward their resume to
gbacon <@t> gispecialists.org


------------------------------

Message: 18
Date: Fri, 23 Mar 2012 07:54:07 -0400
From: marc centofante <marccentofante <@t> gmail.com>
Subject: [Histonet] Post question
To: histonet <@t> lists.utsouthwestern.edu
Message-ID:
	<CALbveuAz3G+EpdnejKBhp7qfO1Dey3B-vUEbw0mNd4kWonrbpg <@t> mail.gmail.com>
Content-Type: text/plain; charset=ISO-8859-1

I am not sure how to post to histonet but if I email this address and then
it gets posted here is what I would like to post:

My employer is looking to buy an anatomic pathology lab with frozen section
service.  Does anyone know of any labs for sale?

-- 
Thank you,
Marc Centofante
marccentofante <@t> gmail.com


------------------------------

Message: 19
Date: Fri, 23 Mar 2012 12:25:27 +0000
From: "James S." <sonya.martin <@t> soton.ac.uk>
Subject: [Histonet] Problems using tomato lectin
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5BE0438A6D85E645A67A4A2F58F41693F639 <@t> UOS-MSG00041-SI.soton.ac.uk>
Content-Type: text/plain; charset="us-ascii"

Hi all,

I've just purchased Texas-Red conjugated tomato lectin from Vector and have been trying it out on some frozen mouse liver sections but cant see anything.

Protocol - fresh frozen sections 8um cut and air dried overnight. Fixed (10min) acetone, washed PBS, tomato lectin applied (I've done serial dilutions from 1/100 - 1/5000 in PBS/0.05% Tween) 1hr at room temp, wash PBS, mount Vectashield + dapi.

Any suggestions?

Thanks
Sonya






------------------------------

Message: 20
Date: Fri, 23 Mar 2012 12:34:01 +0000
From: "James S." <sonya.martin <@t> soton.ac.uk>
Subject: [Histonet] Mouse abs on mouse tissue method
To: "'histonet <@t> lists.utsouthwestern.edu'"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<5BE0438A6D85E645A67A4A2F58F41693F64A <@t> UOS-MSG00041-SI.soton.ac.uk>
Content-Type: text/plain; charset="us-ascii"

I've been trying out the method from IHC world to use mouse primary plus anti-mouse secondary abs on mouse tissue.

Protocol;

Fresh frozen sections (mouse spleen) cut 8um and air dried
Fixed acetone 10min
Wash PBS
Block with 2.5% normal goat serum in PBS/Tw, 30min
Block with unconjugated Fab fragment goat anti-mouse IgG (Jackson Immuno, diluted 1/10 in PBS/Tw), 2hrs room temp
Wash PBS/Tw
Seconary ab - DyLight488 conjugated Fab fragment goat anti-mouse IgG (Jackson Immuno, 1/500 PBS/Tw), 20min room temp
Wash PBS/Tw
Mount

Blocking with unconjugated Fab did decrease background if I used an IgG goat anti-mouse secondary but the background was actually increased when I used the Fab goat anti-mouse!!

Any suggestions?

Thanks
Sonya



------------------------------

Message: 21
Date: Fri, 23 Mar 2012 05:42:33 -0700 (PDT)
From: Bernadette del Rosario <badzrosari <@t> yahoo.com>
Subject: [Histonet] PROTOCOL FOR COLOR CODING BIOPSY CASSETTES
To: "histonet <@t> lists.utsouthwestern.edu"
	<histonet <@t> lists.utsouthwestern.edu>
Message-ID:
	<1332506553.66638.YahooMailNeo <@t> web130204.mail.mud.yahoo.com>
Content-Type: text/plain; charset=iso-8859-1

Good day histonetters.We are a  new university hospital and setting up histopatholology lab.I used to work with white biopsy cassettes only but not technicolors.Got this boss who ask me protocols on colored cassettes etc...No idea about this.Is there any standard pattern  which i can just base and copy (example skin-yellow;breast-pink etc..)Im trying to surf in the net but cant find..Please someone help me???

------------------------------

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