[Histonet] RE: Fixation for breasts

[Tony Henwood (SCHN)]

Try Microwaving blocks in 10% NBF or alcoholic formalin for 2 hours at 45oC.

Works a treat with placentas

Regards 
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) 
Laboratory Manager & Senior Scientist 
Tel: 612 9845 3306 
Fax: 612 9845 3318 
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA 


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Mandy O'Connor
Sent: Tuesday, 20 March 2012 2:37 AM
To: 'histonet <@t> lists.utsouthwestern.edu'
Subject: [Histonet] Fixation for breasts

Hey fellow histo nuts!  Anyone have any good recommendations for fixing breast tissue?  We were wondering about an alcoholic formalin but are unsure if it will hinder or mess up the SISH results.

We usually end up re-processing the block again just to get a good slide that is semi readable.  Thanks for any and all help!

Mandy O'Connor  HTL(ASCP)
Yellowstone Pathology Instit.
Billings, MT
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