[Histonet] questionaire about fixation

[Rene J Buesa]

As long as you a line the tissue is NOT EVEN penetrated and much less fixed.
René J.

--- On Mon, 3/19/12, Emily Sours <talulahgosh <@t> gmail.com> wrote:


From: Emily Sours <talulahgosh <@t> gmail.com>
Subject: Re: [Histonet] questionaire about fixation
To: histonet <@t> lists.utsouthwestern.edu
Date: Monday, March 19, 2012, 12:29 PM


So if you have a line, doesn't that mean your tissue isn't fixed properly?


The whole point of this country is if you want to eat garbage, balloon up
to 600 pounds and die of a heart attack at 43, you can! You are free to do
so. To me, that’s beautiful.
--Ron Swanson



On Mon, Mar 19, 2012 at 12:26 PM, Rene J Buesa <rjbuesa <@t> yahoo.com> wrote:

> Emily:
> With formalin fixation, the formalin advances within the tissue in a way
> that you can macroscopically see up to where the formalin has penetrated.
> The appearance of the tissue changes in color and consistence. The change
> in appearance goes from the exterior to the interior of the tissue and that
> advance is seen as a line, hence "the front line" of the penetration.
> René J.
>
> --- On *Sun, 3/18/12, Emily Sours <talulahgosh <@t> gmail.com>* wrote:
>
>
> From: Emily Sours <talulahgosh <@t> gmail.com>
> Subject: Re: [Histonet] questionaire about fixation
> To: histonet <@t> lists.utsouthwestern.edu
> Date: Sunday, March 18, 2012, 10:23 PM
>
>
> What the heck is a frontline?
> I tried to google it, but I got nothing useful.
>
> The whole point of this country is if you want to eat garbage, balloon up
> to 600 pounds and die of a heart attack at 43, you can! You are free to do
> so. To me, that’s beautiful.
> --Ron Swanson
>
>
>
> On Sun, Mar 18, 2012 at 2:38 PM, Gudrun Lang <gu.lang <@t> gmx.at<http://us.mc1621.mail.yahoo.com/mc/compose?to=gu.lang@gmx.at>>
> wrote:
>
> > Dear histonet!
> >
> > A few hours after submersion of a tissue-block (eg liver) in NBF, the
> block
> > is cut across and we see the colour-change from brown to grey at the
> > margins
> > until a frontline.
> >
> > This frontline shows us:
> >
> > a) the distance of penetration of NBF;
> >
> > b) the distance of penetration AND addition of methylol-groups;
> >
> > c) the distance of penetration AND addition of methylol-groups AND (at
> > least
> > beginning) cross-linking.
> >
> >
> >
> > Do you see the issue?
> >
> > I would be happy, if you can help me to find a correct answer.
> >
> >
> >
> > Regards
> >
> > Gudrun Lang
> >
> >
> >
> > Biomed. Analytikerin
> >
> > Histolab Linz, Austria
> >
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> >
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