[Histonet] IHC Processor Validation & MUM1

[Kim Donadio]

CAP/ASCO recommends at least 25 cases for ER, PR and Her2.  I just did this a few months ago. As far as the rest verify that each of the ab you are doing work and compare them to what you were doing before. How many it takes to sastify your pathologist that the others are working and they sign off on them should be ok. Hope this helps. 
Kim D

Sent from my iPhone

On Mar 13, 2012, at 9:29 AM, "Bliven, Laura" <bliven.laura <@t> marshfieldclinic.org> wrote:

> 
> 1. Any feedback on the process for validating a new tissue processor? Finding 80 to 100 breast specimens that are large enough to divide up and retest Estrogen and Progesterone would take a long time to complete. Also, how many antibodies and specimens should be tested? As we all know, some tumors are very rare and it's only after they are processed that we really know their diagnosis. We will be keeping some current processors, just adding a new one.
> 
> 2. Also, any feedback on clones for MUM1? The antibody we are currently validating is staining some follicular lymphomas which should generally be negative. I do not feel the antigen retrieval is too harsh and believe it may be the clone.
> 
> Thanks,
> Laura
> 
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