[Histonet] Thick Sections cut at 8 microns and up

[Rittman, Barry R]

Bharti
One thing that usually works is to have a thin layer of a softer melting point wax on the top and bottom of the block, this should allow the sections to adhere to each other and not roll up. Need only a 5 degree or so difference in the wax for this to work.
Another is to use a slightly lower melting point wax for your embedding.
Barry

________________________________________
From: histonet-bounces <@t> lists.utsouthwestern.edu [histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Bharti Parihar [bhartologist <@t> gmail.com]
Sent: Saturday, March 10, 2012 10:03 PM
To: Histonet Archive
Subject: [Histonet] Thick Sections cut at 8 microns and up

Hello Hitsonetters! I worked on a lab today for my Histology program which
called for cutting brain sections at 12 microns. The lab we are doing is
Weils to demonstrate myelin. Man oh man was it hard to cut 12 micron
sections. Apparently 10 microns work for this stain as well which I managed
to do that more easily than 12 but I used the idea of using a small brush
to help guide the section down the knife plate like when cutting frozen
sections so it wouldn't instantly roll up into a tube which is what the 12
micron sections kept doing. Any tricks to cutting thick sections?
Suggestions for the student here? Thanks.
-Bharti Parihar
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