[Histonet] mesocolon lymphnode treatment with aceton

[Gudrun Lang]

Dear all!

Does anyone out there use this method for mesocolon clearing with aceton? I
heard only a few facts about it and would like to hear about personal
experience.

The mesocolon is first fixed in NBF, then put in pure aceton, then treated
with a teasing roller and put in a "pipe" with holes. The tissue is
compressed to squeeze out the fat to form a kind of sausage.

The sausage is sliced up, the slices are put into cassettes and processed.

I don't know the exact details. If you have the access to this article, you
can read more (I don't have). 

http://journals.lww.com/ajsp/Abstract/2012/02000/Optimal_Lymph_Node_Harvest_
in_Rectal_Cancer__UICC.5.aspx

or this:

http://www.springerlink.com/content/748617k331l06143/

 

thank you

 

Gudrun Lang

 

Biomed. Analytikerin

Histolab, Linz, Austria