[Histonet] RE: xylene substitute for processing

[Jennifer MacDonald]

One thing to take into consideration that many of the xylene substitutes 
take longer to clear than xylene.  You may have to adjust your processing 
schedule.  We use a xylene substitute with success.




"Marcum, Pamela A" <PAMarcum <@t> uams.edu> 
Sent by: histonet-bounces <@t> lists.utsouthwestern.edu
03/07/2012 07:58 AM

To
"'Masood, Sameena Kathryn'" <sameena.k.masood <@t> Vanderbilt.Edu>, "Cross, 
Kelly" <KCross <@t> cvm.tamu.edu>, "Histonet Listserv (E-mail) 
(histonet <@t> lists.utsouthwestern.edu)" <histonet <@t> lists.utsouthwestern.edu>
cc

Subject
[Histonet] RE: xylene substitute for processing






Some people have issues with the smell and the other option is Anatech 
ProPar or Richard Allan Thermo non-aliphatic hydrocarbon subs which have 
very low odor and are also about the same price.  We can't use the orange 
smell in our lab as some people have allergies and others just don't like 
it.  Both work types work very well and it is a lab choice for the 
options, not a quality choice.  Be aware most processor companies still 
require xylene in the clean cycle to keep your system healthy.

Pam Marcum

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Masood, 
Sameena Kathryn
Sent: Wednesday, March 07, 2012 9:36 AM
To: Cross, Kelly; Histonet Listserv (E-mail) 
(histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] RE: xylene substitute for processing

Kelly,

Histo-Clear can be directly substituted for Xylene at every point in 
processing and staining.  It's completely non-toxic (even has a food grade 
rating), is made from oranges, and smells delicious. You don't have to 
work in a hood and it seems to make tissue less brittle than Xylene also. 
Some guys in our Histology Core told us about it and we have been happy 
with it. You can purchase it from the manufacturer, National Diagnostics, 
or Fisher.

http://www.nationaldiagnostics.com/product_info.php?products_id=175

Good luck,

Kathryn





-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu 
[mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cross, 
Kelly
Sent: Monday, March 05, 2012 10:24 AM
To: Histonet Listserv (E-mail) (histonet <@t> lists.utsouthwestern.edu)
Subject: [Histonet] xylene substitute for processing

Greetings Histonet!

Does anyone use xylene substitutes for routine over-night processing? If 
so, what do you use and does it have any adverse effect your special 
stains?

Thank you in advance for your help,
Kelly

Kelly S. Cross B.S., HT (ASCP)
Medical Laboratory Supervisor
Veterinary Pathobiology
Texas Veterinary Medical Center
Texas A&M University
College Station, TX 77843-4467
979-862-3658 Office
979-845-5149 Lab

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