[Histonet] RE: xylene substitute for processing

[Grantham, Andrea L - (algranth)]

I have been using Clear Rite 3 for years on animal tissue in a VIP for overnite processing and it is great. I get it from Fisher. There has never been a problem with special stains but I use xylene on my stain lines.

Andi Grantham




On Mar 7, 2012, at 8:35 AM, Masood, Sameena Kathryn wrote:

> Kelly,
> 
> Histo-Clear can be directly substituted for Xylene at every point in processing and staining.  It's completely non-toxic (even has a food grade rating), is made from oranges, and smells delicious. You don't have to work in a hood and it seems to make tissue less brittle than Xylene also. Some guys in our Histology Core told us about it and we have been happy with it. You can purchase it from the manufacturer, National Diagnostics, or Fisher.
> 
> http://www.nationaldiagnostics.com/product_info.php?products_id=175
> 
> Good luck,
> 
> Kathryn
> 
> 
> 
> 
> 
> -----Original Message-----
> From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Cross, Kelly
> Sent: Monday, March 05, 2012 10:24 AM
> To: Histonet Listserv (E-mail) (histonet <@t> lists.utsouthwestern.edu)
> Subject: [Histonet] xylene substitute for processing
> 
> Greetings Histonet!
> 
> Does anyone use xylene substitutes for routine over-night processing? If so, what do you use and does it have any adverse effect your special stains?
> 
> Thank you in advance for your help,
> Kelly
> 
> Kelly S. Cross B.S., HT (ASCP)
> Medical Laboratory Supervisor
> Veterinary Pathobiology
> Texas Veterinary Medical Center
> Texas A&M University
> College Station, TX 77843-4467
> 979-862-3658 Office
> 979-845-5149 Lab
> 
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