[Histonet] RE: Destain/Restain

[Tom McNemar]

We do not destain h&e slides.  We just take them back to water and stain right over top of the h&e.

Tom McNemar, HT(ASCP)
Histology Co-ordinator
Licking Memorial Health Systems
(740) 348-4163
(740) 348-4166
tmcnemar <@t> lmhealth.org
www.LMHealth.org


-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of Virginia Chladek
Sent: Monday, March 05, 2012 3:39 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] Destain/Restain

****************************************

I need your help-

My doc needs me to destain an h&e slide to run a ck5 on the Bond3, how
can I do this without the tissue falling off? I ran the slide back down
from xylene to water and destained, then introduced the slide to bond
wash before starting the protocol in bond wash again and lost the
tissue. Any suggestions?

Thanks!
Maggie

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