[Histonet] glacial acetic acid vs bouins

[Gill, Caula A.]

What works best for us is a 10% acetic acid solution. We mix up a gallon whenever needed and dip large tissues in the solution. For smaller sections of tissue a squirt bottle works well.
Hope this helps . 

-----Original Message-----
From: histonet-bounces <@t> lists.utsouthwestern.edu [mailto:histonet-bounces <@t> lists.utsouthwestern.edu] On Behalf Of angela smith
Sent: Monday, March 05, 2012 8:33 PM
To: histonet <@t> lists.utsouthwestern.edu
Subject: [Histonet] glacial acetic acid vs bouins 

I know many years ago we used a dilute glacial ac. acid to "dip" our tissues in that had blue or black dyes applied to help the dye stay on the tissue. Presently we use bouins. We want to discontinue the use of bouins for this. Does anyone out there remember what dilution works best?  

Thanks
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