[Histonet] replacing tween in the secondary hyb.solution

[Geert van Haalem]

Hi,

I currently did an experiment in which I left out Tween in de hybridisation solution of
the secondary antibodies. I only made a solution of two Alexa in PBS. When imaging
Bye eye I did not notice any decrease in signal compared to a hyb.solution with Tween.
My intention is to replace tween with FBS, but can anybody tell me pro and cons for
this idea.

With regards,

Geert