[Histonet] RE: Congo red

[Jerry Ricks]

>From the CAP site: 

"The Benhold Congo red technique does not yield reproducible results and should be avoided"

and 

"the alkaline Congo red method of Puchtler, et al.,7 remains the gold standard for the demonstration of amyloid in tissue sections.
"



http://www.cap.org/apps/portlets/contentViewer/show.do?printFriendly=true&contentReference=cap_today%2F0609%2F0609g_histologic_preparations.html


Jerry



> From: cmiller <@t> physlab.com
> To: histonet <@t> lists.utsouthwestern.edu
> CC: histonet-bounces <@t> lists.utsouthwestern.edu
> Date: Thu, 1 Mar 2012 08:28:18 -0600
> Subject: [Histonet] Congo red
> 
> Anyone have any solution to a week Congo Red? I use Rowley  Congo red, 1% aqueous order # S0-496. our procedure is Benholds and I cut at 5-6 microns. And I leave in the solution for up to 4 hours and the paths are still saying it is weak. Any ideas? I have even stopped the Alkaline alcohol differentiation step and its still too weak.
> 
> Cheryl A. Miller HT(ASCP)cm
> Histology/Cytology Prep Supervisor
> Physicians Laboratory Services
> Omaha, NE. 402 731 4145 ext. 554
> 
> 
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