[Histonet] blocking endogenous activities

[Tuyen Nguyen]

In my lab, in IHC protocols for most antibodies, hydrogen peroxide is applied before antigen heat retrieval. However, I wonder there is any different result in IHC stain if hydrogen peroxide for blocking endogenous activities is applied after antigen heat retrieval by using steamer or pressure cooker?
Thank you,
Mai Nguyen Truong
Research Associate II
Los Angeles, California